8IR4
Crystal structure of the SLF1 BRCT domain in complex with a Rad18 peptide containing pS442
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-18 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.421, 76.098, 141.371 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 1.620 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.340 | 1.710 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.093 | 0.715 |
| Number of reflections | 55039 | 7782 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 98.0 | 96.5 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.997 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.3M MgCl2, 0.1M BIS-TRIS pH6.5, 22% PEG3350 |
