8IR2
Crystal structure of the SLF1 BRCT domain in complex with a Rad18 peptide containing pS442 and pS444
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.352, 76.203, 141.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.100 - 1.750 |
R-factor | 0.1658 |
Rwork | 0.164 |
R-free | 0.20230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.890 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
Rmerge | 0.170 | 0.057 | 1.126 |
Rmeas | 0.178 | 0.060 | 1.206 |
Rpim | 0.051 | 0.017 | 0.428 |
Total number of observations | 520523 | ||
Number of reflections | 44776 | 2452 | 2159 |
<I/σ(I)> | 3.7 | ||
Completeness [%] | 100.0 | 99.9 | 99.5 |
Redundancy | 11.6 | 11.8 | 7.9 |
CC(1/2) | 0.999 | 0.999 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.19M MgCl2, 0.1M BIS-TRIS pH6.5, 25% PEG3350 |