8IR0

AfFer mutant-P156F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	I 4 2 2
Unit cell lengths	116.513, 116.513, 206.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.650 - 2.890
R-factor	0.1902
Rwork	0.185
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.120
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.650	2.950
High resolution limit [Å]	2.890	2.900
Rmerge		0.519
Number of reflections	16300	16300
<I/σ(I)>	2
Completeness [%]	99.7
Redundancy	12.7
CC(1/2)	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MES, 20%(v/v) 1,4-butanediol, 200 mM Lithium sulfate, pH 6.0