8IQZ
Asterias forbesii ferritin mutant-P156F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 160.713, 160.713, 199.729 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.550 - 4.190 |
| R-factor | 0.2188 |
| Rwork | 0.214 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.461 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.18.2-3874) |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.050 | 4.270 |
| High resolution limit [Å] | 4.190 | 4.200 |
| Rmerge | 0.691 | |
| Number of reflections | 22303 | 119885 |
| <I/σ(I)> | 1.3 | |
| Completeness [%] | 99.2 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Tris, pH9.0, 1.0 M NaCl |
