8IQY

Asterias forbesii ferritin mutant-P156H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	I 4 2 2
Unit cell lengths	116.934, 116.934, 206.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.560 - 2.100
R-factor	0.1654
Rwork	0.165
R-free	0.18290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.788
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.560	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	42226	42226
<I/σ(I)>	2.7
Completeness [%]	99.7
Redundancy	13
CC(1/2)	0.943	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM imidazole, 15%(v/v) reagent alcohol, 200 mM magnesium chloride