8IQS
Crystal structure of Anti-PEG antibody M11 Fv-clasp fragment with PEG (co-crystallization with PEG3350)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.330, 103.460, 174.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.840 - 2.160 |
R-factor | 0.2374 |
Rwork | 0.236 |
R-free | 0.27040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.467 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.990 | 2.290 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmeas | 0.078 | 2.293 |
Number of reflections | 46727 | 7406 |
<I/σ(I)> | 18.55 | 0.91 |
Completeness [%] | 100.0 | |
Redundancy | 9.47 | |
CC(1/2) | 0.999 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium sulfate, 20% (w/v) PEG 3350 |