8IQQ
Crystal structure of Anti-PEG antibody M9 Fv-clasp fragment with PEG (co-crystallization with PEG2000MME)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.990, 70.250, 103.580 |
Unit cell angles | 90.00, 110.94, 90.00 |
Refinement procedure
Resolution | 48.370 - 2.020 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.500 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.140 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmeas | 0.231 | 2.293 |
Number of reflections | 48467 | 7637 |
<I/σ(I)> | 5.27 | 1.05 |
Completeness [%] | 99.8 | |
Redundancy | 6.5 | |
CC(1/2) | 0.990 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl pH 7.0 and 24% (w/v) PEG 2000MME |