8IQP
Crystal structure of Anti-PEG antibody M9 Fv-clasp fragment with PEG (co-crystallization with PEG3350)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.230, 68.340, 94.790 |
Unit cell angles | 90.00, 104.20, 90.00 |
Refinement procedure
Resolution | 45.950 - 1.760 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.896 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.950 | 1.860 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmeas | 0.105 | 1.415 |
Number of reflections | 71343 | 11764 |
<I/σ(I)> | 5.54 | 0.55 |
Completeness [%] | 99.6 | |
Redundancy | 3.22 | |
CC(1/2) | 0.995 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium acetate and 25% (w/v) PEG3350 |