8IQM
Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-19 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.465, 56.294, 62.673 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.337 - 1.967 |
R-factor | 0.1999 |
Rwork | 0.196 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.521 |
Data reduction software | autoPROC |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.340 | 2.037 |
High resolution limit [Å] | 1.967 | 1.967 |
Number of reflections | 21592 | 1172 |
<I/σ(I)> | 2.77 | |
Completeness [%] | 98.0 | |
Redundancy | 8.5 | |
CC(1/2) | 0.996 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M potassium thiocyanate, 20% polyethylene glycol monomethyl ether 2,000. |