8IQJ

Crystal structure of SARS-CoV2 N-NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-14
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	58.801, 93.125, 97.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.2365
Rwork	0.232
R-free	0.28880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.079	0.435
Number of reflections	23778	2411
<I/σ(I)>	20.772	3.77
Completeness [%]	96.6	99.8
Redundancy	6.2	5.8
CC(1/2)		0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.005 M Spermine tetrahydrochloride, 0.05 M Potassium Chloride, 0.05 M Bis-Tris HCl pH 7.0, 31 % PEG 3350