8IQD
Crystal structure of AsfvPrimPol N-terminal Prim/Pol domain in complex with Mn2+ and dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 209.160, 42.814, 128.214 |
Unit cell angles | 90.00, 125.04, 90.00 |
Refinement procedure
Resolution | 85.620 - 2.390 |
R-factor | 0.244 |
Rwork | 0.241 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold |
RMSD bond length | 0.003 |
RMSD bond angle | 0.601 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.310 | 2.520 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.075 | 0.530 |
Rmeas | 0.091 | 0.633 |
Rpim | 0.051 | 0.343 |
Total number of observations | 115898 | 17416 |
Number of reflections | 36675 | 5290 |
<I/σ(I)> | 8.9 | 2.4 |
Completeness [%] | 97.6 | |
Redundancy | 3.2 | 3.3 |
CC(1/2) | 0.991 | 0.827 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M SPG buffer pH 8.0 and 25% PEG 1500 |