8IPM
The structure of human mitochondrial methyltransferase METTL15 with h44_RNA, RBFA and SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.220, 134.111, 93.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.528 - 3.100 |
| R-factor | 0.2564 |
| Rwork | 0.253 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.537 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.530 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.105 | 0.616 |
| Number of reflections | 8176 | 888 |
| <I/σ(I)> | 14.7 | 3.7 |
| Completeness [%] | 97.4 | |
| Redundancy | 9 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 4000, 0.2 M ADA (pH 6.4) |
