8IP2

Escherichia coli OpgG mutant-D361N with beta-1,2-glucan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	62.821, 80.970, 213.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.340 - 1.810
Rwork	0.213
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.719
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.340	1.850
High resolution limit [Å]	1.810	1.810
Number of reflections	50021	2971
<I/σ(I)>	17.2
Completeness [%]	99.9
Redundancy	6.4
CC(1/2)	0.999	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MMT (pH 5.0), PEG400