8IOX
Escherichia coli OpgD mutant-D388N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 226.750, 392.760, 324.648 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.143 - 2.950 |
Rwork | 0.194 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.602 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.143 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.208 | |
Number of reflections | 301232 | 14824 |
<I/σ(I)> | 13 | |
Completeness [%] | 100.0 | |
Redundancy | 13.7 | |
CC(1/2) | 0.994 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG2000MME, TMAO, tris-HCl (pH 8.5) |