8IOM
Crystal structure of the carboxy-terminal channel-forming domain of Colicin Ib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2021-10-28 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | I 41 |
Unit cell lengths | 91.994, 91.994, 113.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.080 - 3.000 |
R-factor | 0.1985 |
Rwork | 0.196 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.544 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.060 |
High resolution limit [Å] | 2.940 | 2.940 |
Rmerge | 0.090 | 0.556 |
Rmeas | 0.101 | 0.627 |
Rpim | 0.047 | 0.289 |
Number of reflections | 11040 | 1107 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.6 | 4.7 |
CC(1/2) | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.5 | Precipitant: 0.1M sodium chloride, 0.1M sodium HEPES pH7.5, 12% w/v PEG 4000, 0.5 microliter 8mg/ml colicin Ib C domain mix with the same volume precipitant in the drop colicin Ib C domain buffer:20mM sodium, citrate pH5.2, 200mM NaCl |