8INP
A reversible glycosyltransferase of tectorigenin - Bc7OUGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.134, 83.395, 160.478 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.120 - 2.120 |
| R-factor | 0.2452 |
| Rwork | 0.242 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.981 |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.167 | 1.528 |
| Number of reflections | 38331 | 3086 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.17M Lithium sulfate monohydrate, 0.085M Tris hydrochloride pH8.5, 25.5% w/v PEG 4000 |
