8INJ

Crystal structure of UGT74AN3-UDP-DIG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	203
Detector technology	PIXEL
Collection date	2021-07-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	45.718, 52.210, 201.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 1.760
R-factor	0.1951
Rwork	0.194
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.870	1.900
High resolution limit [Å]	1.760	1.860
Number of reflections	48873	2603
<I/σ(I)>	17.1
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG2000MME, 0.1M Hepes (7.5), 0.2M potassium nitro