8IND

Crystal structure of UGT74AN3-UDP-RES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	203
Detector technology	PIXEL
Collection date	2021-06-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	45.995, 52.911, 201.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.460 - 1.850
R-factor	0.1842
Rwork	0.182
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	XDS (1.19.2_4158)
Data scaling software	Aimless
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.460	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.100
Number of reflections	42938	2638
<I/σ(I)>	15
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.997	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG2000MME, 0.1M Hepes (7.5), 0.2M potassium nitro