8IN7

Crystal structure of UGT74AN3-UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	45.437, 52.068, 200.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.190 - 1.900
R-factor	0.1864
Rwork	0.184
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.190	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	38464
<I/σ(I)>	25.2
Completeness [%]	82.9
Redundancy	12.4
CC(1/2)	0.998	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG2000MME, 0.1M Hepes (7.5), potassium nitro