8IN6

Eisenia hydrolysis-enhancing protein from Aplysia kurodai complex with tannic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	42.463, 65.356, 67.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.890 - 1.900
R-factor	0.1951
Rwork	0.193
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.987
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.840	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.082	0.823
Number of reflections	15284	1493
<I/σ(I)>	15.39
Completeness [%]	99.9	99.73
Redundancy	6.4	6.4
CC(1/2)	0.999	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0 M sodium acetate, 0.1 M imidazole (pH 6.5)