8IN6
Eisenia hydrolysis-enhancing protein from Aplysia kurodai complex with tannic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.463, 65.356, 67.173 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.890 - 1.900 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.987 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.840 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.082 | 0.823 |
Number of reflections | 15284 | 1493 |
<I/σ(I)> | 15.39 | |
Completeness [%] | 99.9 | 99.73 |
Redundancy | 6.4 | 6.4 |
CC(1/2) | 0.999 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M sodium acetate, 0.1 M imidazole (pH 6.5) |