8IN1

beta-glucosidase protein from Aplysia kurodai

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 62
Unit cell lengths	191.669, 191.669, 112.551
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.650 - 2.700
R-factor	0.1849
Rwork	0.183
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.650	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	64457	6309
<I/σ(I)>	10.69	2.57
Completeness [%]	99.9
Redundancy	10.7
CC(1/2)	0.995	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate pH 4.5, and 20% PEG 3350