8IMS
Crystal structure of TRAF7 coiled-coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978530 |
Spacegroup name | P 64 |
Unit cell lengths | 169.923, 169.923, 57.055 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.090 - 3.300 |
R-factor | 0.2624 |
Rwork | 0.262 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.661 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.090 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 14379 | 1393 |
<I/σ(I)> | 8.22 | |
Completeness [%] | 99.8 | |
Redundancy | 4.07 | |
CC(1/2) | 0.996 | 0.527 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES pH6.9, 0.2M magnesium formate dihydrate |