8IMR

Structure of ligand-free human macrophage migration inhibitory factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-22
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	67.745, 68.471, 88.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.170 - 1.300
R-factor	0.1964
Rwork	0.195
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.737
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.350
High resolution limit [Å]	1.300	1.300
Number of reflections	95814	10054
<I/σ(I)>	21.4	2.2
Completeness [%]	93.8	99.7
Redundancy	6.3	6.1
CC(1/2)	0.993	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	283	2.0 M Ammonium sulfate 0.1 M Tris-HCl (pH 8.0) 4 % Isopropanol