8IMG
Human cGAS catalytic domain bound with C20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 214.057, 46.045, 86.849 |
Unit cell angles | 90.00, 113.94, 90.00 |
Refinement procedure
Resolution | 35.680 - 1.800 |
R-factor | 0.2244 |
Rwork | 0.223 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.810 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.620 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.137 | 2.289 |
Rmeas | 0.150 | 2.509 |
Rpim | 0.060 | 1.011 |
Number of reflections | 72429 | 5327 |
<I/σ(I)> | 6.8 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 6.3 | 6.3 |
CC(1/2) | 0.989 | 0.353 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 18-20% V/V PEG 3350, 0.2 M AMMONIUM CITRATE |