8IMD
Crystal structure of Cu/Zn Superoxide dismutase from Paenibacillus lautus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.275 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.240, 53.612, 169.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.350 - 1.450 |
| R-factor | 0.18988 |
| Rwork | 0.188 |
| R-free | 0.22058 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.766 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.310 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.054 | 1.538 |
| Number of reflections | 74112 | 10562 |
| <I/σ(I)> | 26.6 | 1.8 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 18.5 | 15.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 22.5% PEG 4,000, 100 mM acetic acid and sodium acetate buffer |
