8IM8
Crystal structure of Periplasmic alpha-amylase (MalS) from E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 133.242, 133.242, 386.269 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.020 - 2.700 |
R-factor | 0.1796 |
Rwork | 0.178 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ea9 5a2a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.211 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.020 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmeas | 0.123 | 0.684 |
Number of reflections | 69932 | 4802 |
<I/σ(I)> | 168 | 5.5 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M Sodium citrate tribasic dihydrate pH5.5 20% w/v Polyehtylene glycol 1000 0.1M Lthium sulfate monohydrate |