8IM1
mCherry-LaM1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-26 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.070, 74.830, 94.640 |
| Unit cell angles | 111.33, 100.68, 104.42 |
Refinement procedure
| Resolution | 32.130 - 2.050 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.691 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.970 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.086 | 0.987 |
| Rmeas | 0.101 | 1.169 |
| Rpim | 0.053 | 0.619 |
| Total number of observations | 349365 | 25139 |
| Number of reflections | 97591 | 7164 |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 96.9 | |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.996 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Potassium sulfate, 20 %(w/v) PEG 3350 |
