8IM0
mCherry-LaM8 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-26 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.075, 75.310, 83.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.360 - 1.310 |
| R-factor | 0.1783 |
| Rwork | 0.178 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.117 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.620 | 1.340 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.062 | 0.880 |
| Rmeas | 0.065 | 0.988 |
| Rpim | 0.019 | 0.430 |
| Total number of observations | 1055879 | 28966 |
| Number of reflections | 95127 | 6239 |
| <I/σ(I)> | 18.5 | 1.6 |
| Completeness [%] | 98.9 | |
| Redundancy | 11.1 | 4.6 |
| CC(1/2) | 0.999 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M magnesium chloride, 0.1 M Tris-HCl (pH 8.5), 30% (w/v) PEG 4000 |
