8ILT
Crystal structure of Est30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 149.964, 59.988, 166.039 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 49.540 - 2.420 |
| R-factor | 0.21699 |
| Rwork | 0.214 |
| R-free | 0.27318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 2.480 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Number of reflections | 112238 | 8256 |
| <I/σ(I)> | 10.78 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.73 | |
| CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Zinc acetate tetrahydrate, PEG3350 |
