8ILT
Crystal structure of Est30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 149.964, 59.988, 166.039 |
Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
Resolution | 49.540 - 2.420 |
R-factor | 0.21699 |
Rwork | 0.214 |
R-free | 0.27318 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.318 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.540 | 2.480 |
High resolution limit [Å] | 2.420 | 2.420 |
Number of reflections | 112238 | 8256 |
<I/σ(I)> | 10.78 | |
Completeness [%] | 100.0 | |
Redundancy | 6.73 | |
CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Zinc acetate tetrahydrate, PEG3350 |