8ILJ
S-formylglutathione hydrolase (BuSFGH) from Burkholderiaceae sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2020-05-05 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.370, 109.290, 112.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.520 - 1.730 |
R-factor | 0.1954 |
Rwork | 0.195 |
R-free | 0.20980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.148 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.520 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.081 | 0.397 |
Number of reflections | 65529 | 6421 |
<I/σ(I)> | 20.63 | |
Completeness [%] | 99.7 | |
Redundancy | 12.5 | |
CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.2 M ammonium citrate pH 5.0 and 20% (w/v) PEG 3350 |