8ILG

The crystal structure of dG-DNA:Pol X product binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-05-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	69.033, 81.422, 92.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.861 - 1.805
R-factor	0.2234
Rwork	0.222
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.136	0.083	0.371
Total number of observations	209940
Number of reflections	84882	9225	7433
<I/σ(I)>	6.1
Completeness [%]	91.6	99.5	80
Redundancy	2.5	3.3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Potassium Formate (0.2 M), 16% PEG3350