8IL9

Crystal structure of the LOV1 Q122N mutant of Klebsormidium nitens phototropin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-29
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.918402
Spacegroup name	P 43 21 2
Unit cell lengths	53.878, 53.878, 137.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 2.160
Rwork	0.184
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.684
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.098	5.847
High resolution limit [Å]	2.156	2.156
Rmerge	0.095
Number of reflections	13135	11392
<I/σ(I)>	21.5
Completeness [%]	98.6	98.6
Redundancy	24.4
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Nickel chloride hexahydrate, Tris pH 8.5, lithium sulfate monohydrate