8IL7
Structure of human soluble Adenylyl Cyclase in complex with lactate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2022-10-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 63 |
Unit cell lengths | 99.280, 99.280, 98.564 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.640 - 1.950 |
R-factor | 0.1642 |
Rwork | 0.163 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.322 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.640 | 2.050 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 40207 | 5705 |
<I/σ(I)> | 20.3 | |
Completeness [%] | 99.9 | |
Redundancy | 14.3 | |
CC(1/2) | 0.998 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 289.15 | 0.2 M Sodium citrate tribasic, 15% PEG 3350, 0.1 M sodium lactate pH 3.8, 10% glycerol |