8IL0
Crystal structure of LmbT from Streptomyces lincolnensis NRRL ISP-5355
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979021 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 103.741, 103.741, 197.251 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.310 - 2.810 |
R-factor | 0.2328 |
Rwork | 0.231 |
R-free | 0.25730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.820 | 2.960 |
High resolution limit [Å] | 2.810 | 2.810 |
Number of reflections | 12924 | 1925 |
<I/σ(I)> | 23 | |
Completeness [%] | 95.3 | |
Redundancy | 10 | |
CC(1/2) | 0.999 | 0.831 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Magnesium acetate tetrahydrate, 0.1 M MES, pH6.5 10 %, w/v PEG 10000 |