8IKX
An Arabidopsis polygalacturonase PGLR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 39.212, 43.540, 63.478 |
Unit cell angles | 94.59, 99.99, 110.55 |
Refinement procedure
Resolution | 31.610 - 1.500 |
R-factor | 0.1591 |
Rwork | 0.158 |
R-free | 0.17920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.052 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (v1.20.1-4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.088 | 0.033 | 0.233 |
Total number of observations | 193031 | ||
Number of reflections | 58730 | 5937 | 4921 |
<I/σ(I)> | 8.9 | ||
Completeness [%] | 92.9 | 96.6 | 80.9 |
Redundancy | 1.7 | 1.8 | 1.4 |
CC(1/2) | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M trimethylamine N-oxide dihydrate, 0.1M Tris pH 8.5, 20% w/v PEG MME 2000 |