8IKX
An Arabidopsis polygalacturonase PGLR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.212, 43.540, 63.478 |
| Unit cell angles | 94.59, 99.99, 110.55 |
Refinement procedure
| Resolution | 31.610 - 1.500 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.17920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.088 | 0.033 | 0.233 |
| Total number of observations | 193031 | ||
| Number of reflections | 58730 | 5937 | 4921 |
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 92.9 | 96.6 | 80.9 |
| Redundancy | 1.7 | 1.8 | 1.4 |
| CC(1/2) | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M trimethylamine N-oxide dihydrate, 0.1M Tris pH 8.5, 20% w/v PEG MME 2000 |
