8IK2
RhlA exhibits dual thioesterase and acyltransferase activities during rhamnolipid biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.629, 63.890, 85.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.757 - 2.151 |
| R-factor | 0.164 |
| Rwork | 0.160 |
| R-free | 0.19970 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.099 | 0.099 |
| Number of reflections | 27004 | 27004 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.4 | 99.43 |
| Redundancy | 12.4 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | sodium/potassium phosphate |
