8IK1
EstL5 in complex of PMSF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2022-11-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.153, 105.153, 128.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.350 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.506 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.150 | 2.470 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.047 | |
| Number of reflections | 29348 | 4241 |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 96.3 | 96.9 |
| Redundancy | 8.7 | 7.8 |
| CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.01 M CoCl2-6H2O, pH 4.8 0.1 M Sodium acetate and 1 M 1,6-Hexanediol |
