8IJU
ATP-dependent RNA helicase DDX39A (URH49delta41)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-22 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.974, 96.606, 59.755 |
Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
Resolution | 37.500 - 1.820 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.859 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.046 | 0.373 |
Rmeas | 0.058 | 0.481 |
Number of reflections | 68537 | 10621 |
<I/σ(I)> | 12.4 | 2.09 |
Completeness [%] | 98.5 | 94.2 |
Redundancy | 2.4 | 2.1 |
CC(1/2) | 0.998 | 0.843 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 mM Na2PO4, 30 % (w/v) PEG3350 |