8IJT
crystal structure of Hyp N135A mutant from Hypoxylon sp. E7406B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-02 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 45.872, 86.230, 102.349 |
Unit cell angles | 77.50, 85.91, 87.69 |
Refinement procedure
Resolution | 31.370 - 2.560 |
R-factor | 0.2031 |
Rwork | 0.201 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold |
RMSD bond length | 0.014 |
RMSD bond angle | 1.778 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.437 | 2.602 |
High resolution limit [Å] | 2.558 | 2.558 |
Number of reflections | 48170 | 2372 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 97.7 | |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 13% PEG4000, 0.1 M Na3Cit, pH5.6, 0.15 M NaCl, 0.01 M MgCl2 |