8IJP
Structure of DNA binding domain of McrBC endonuclease bound to DNA: L68Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.069, 68.910, 143.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.150 - 1.550 |
R-factor | 0.1615 |
Rwork | 0.158 |
R-free | 0.19240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sse |
RMSD bond length | 0.013 |
RMSD bond angle | 1.252 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.910 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.076 | 0.869 |
Number of reflections | 53142 | 2591 |
<I/σ(I)> | 15.7 | 2.8 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 12.1 | 12.3 |
CC(1/2) | 0.999 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.1-0.2 M Bis-Tris pH 5.5 and 12-24% PEG 4000 |