8IIU
anti-VEGF nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 31.468, 39.369, 39.952 |
Unit cell angles | 102.14, 90.23, 90.95 |
Refinement procedure
Resolution | 24.590 - 1.270 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.18690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5imk |
RMSD bond length | 0.005 |
RMSD bond angle | 0.910 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.590 | 1.315 |
High resolution limit [Å] | 1.270 | 1.270 |
Number of reflections | 46593 | 4515 |
<I/σ(I)> | 16 | |
Completeness [%] | 94.0 | |
Redundancy | 3.4 | |
CC(1/2) | 0.974 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | (NH4)2SO4, HEPES (pH=7.5), NDSB-195 |