8IIO
H109Q mutant of uracil DNA glycosylase X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-04-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.690, 51.140, 54.580 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 35.580 - 1.670 |
| R-factor | 0.1848 |
| Rwork | 0.184 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.860 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.780 | 1.690 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.054 | 0.418 |
| Rmeas | 0.070 | 0.553 |
| Rpim | 0.044 | 0.358 |
| Number of reflections | 22831 | 940 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 98.9 | 79.8 |
| Redundancy | 4.4 | 3.8 |
| CC(1/2) | 0.996 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
