8IIM
H109K mutant of uracil DNA glycosylase X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-02-27 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.450, 51.750, 54.630 |
| Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
| Resolution | 26.410 - 1.600 |
| R-factor | 0.1648 |
| Rwork | 0.164 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.012 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.810 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | 0.119 |
| Rmeas | 0.045 | 0.161 |
| Rpim | 0.027 | 0.107 |
| Number of reflections | 25154 | 834 |
| <I/σ(I)> | 21.9 | 5.5 |
| Completeness [%] | 96.6 | 65 |
| Redundancy | 4.6 | 2.9 |
| CC(1/2) | 0.998 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |
