8IIJ
H109G mutant of uracil DNA glycosylase X
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-09-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.710, 50.580, 54.830 |
Unit cell angles | 90.00, 104.22, 90.00 |
Refinement procedure
Resolution | 24.350 - 1.830 |
R-factor | 0.1854 |
Rwork | 0.183 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.104 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.100 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.092 | 0.815 |
Rmeas | 0.117 | 1.036 |
Rpim | 0.071 | 0.634 |
Number of reflections | 16704 | 978 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 96.9 | 93.6 |
Redundancy | 5 | 5 |
CC(1/2) | 0.996 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |