8IIH
H109C mutant of uracil DNA glycosylase X
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-02-28 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.380, 51.510, 54.900 |
Unit cell angles | 90.00, 104.67, 90.00 |
Refinement procedure
Resolution | 26.560 - 2.130 |
R-factor | 0.1745 |
Rwork | 0.172 |
R-free | 0.22170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.973 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.060 | 2.190 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.099 | 0.345 |
Rmeas | 0.137 | 0.470 |
Rpim | 0.095 | 0.317 |
Number of reflections | 10487 | 899 |
<I/σ(I)> | 6.8 | 2.3 |
Completeness [%] | 94.2 | |
Redundancy | 3.1 | |
CC(1/2) | 0.989 | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |