8IIG
Complex form of MsmUdgX H109A mutant and uracil- obtained from uracil DNA (ttUtt) post its cleavage by UdgX H109A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2021-09-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.710, 50.720, 55.060 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 26.650 - 2.300 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.610 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.110 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.140 | 0.430 |
Rmeas | 0.190 | 0.590 |
Rpim | 0.130 | 0.390 |
Number of reflections | 8832 | 848 |
<I/σ(I)> | 5.6 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.982 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 295 | 2.0M Ammonium citrate tribasic pH7.0, 0.1M BIS-TRIS propane pH7.0 |