8IIC

Crystal structure of Israeli acute paralysis virus RNA-dependent RNA polymerase delta40 mutant (residues 41-546)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 62 2 2
Unit cell lengths	167.926, 167.926, 218.321
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.780 - 3.250
R-factor	0.2131
Rwork	0.211
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8iib
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.800	23.800	3.370
High resolution limit [Å]	3.250	6.950	3.250
Rmerge	0.071	0.028	0.841
Rmeas	0.075	0.029	0.883
Rpim	0.023	0.009	0.266
Total number of observations	295009
Number of reflections	28880	3064	2858
<I/σ(I)>	8.4
Completeness [%]	98.8	97.1	99.5
Redundancy	10.2	9.4	10.5
CC(1/2)		0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	Ammonium sulfate