8IIA

Crystal structure of the oligomeric state of the extracellular domain of human myelin protein zero(MPZ/P0)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	I 4 2 2
Unit cell lengths	88.579, 88.579, 88.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.270 - 2.090
R-factor	0.2174
Rwork	0.217
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1neu
RMSD bond length	0.005
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.490	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.141	3.297
Rpim	0.028	0.634
Number of reflections	10861	815
<I/σ(I)>	13.9	1.1
Completeness [%]	100.0	99.5
Redundancy	26.8	27.4
CC(1/2)	0.998	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100 mM Tris-HCl, 300 mM magnesium-formate