8II4
Crystal structure of V30M-TTR in complex with 6-hydroxy BBM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.915 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.264, 85.656, 63.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.437 - 1.499 |
R-factor | 0.1835 |
Rwork | 0.183 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.058 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.620 | 1.550 |
High resolution limit [Å] | 1.499 | 1.499 |
Rmeas | 0.080 | 0.733 |
Number of reflections | 38780 | 3764 |
<I/σ(I)> | 14.3 | 2.9 |
Completeness [%] | 99.8 | |
Redundancy | 7.3 | |
CC(1/2) | 0.997 | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 400, CaCl2, Sodium acetate |