8IHX
X-ray crystal structure of N372D mutant of endo-1,4-beta glucanase from Eisenia fetida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.304, 71.614, 55.097 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 48.760 - 1.600 |
| R-factor | 0.1479 |
| Rwork | 0.147 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.832 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.086 | 0.491 |
| Number of reflections | 49763 | 2407 |
| <I/σ(I)> | 10 | 2.7 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG3350, 0.2 M magnesium chloride, 50 mM sodium acetate pH 5.0 |
